7ADV
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI XZ447 (compound 6v)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97957 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 158.610, 158.610, 122.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.160 - 2.650 |
R-factor | 0.1971 |
Rwork | 0.196 |
R-free | 0.21910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bdz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.523 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_3900) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.930 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.086 | 1.701 |
Rpim | 0.027 | 0.555 |
Number of reflections | 45907 | 3274 |
<I/σ(I)> | 20.8 | 1.5 |
Completeness [%] | 99.7 | |
Redundancy | 11.6 | |
CC(1/2) | 0.999 | 0.521 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5 |