7AD6

Crystal structure of human complement C5 in complex with the K92 bovine knob domain peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	204.265, 104.285, 154.880
Unit cell angles	90.00, 124.90, 90.00

Refinement procedure

Resolution	83.770 - 2.750
R-factor	0.2211
Rwork	0.219
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hcc
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX (1.18-3845-000)
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.770	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.019	0.204
Number of reflections	138366	6877
<I/σ(I)>	28.79
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M bicine/Trizma (pH 8.5), 10 % (w/v) PEG 8000, 20 % (v/v) ethylene glycol, 30 mM sodium fluoride, 30 mM sodium bromide and 30 mM sodium iodide.