7ABV
Structure of the S1-cleaved mouse Notch1 Negative Regulatory Region (NRR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-01 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 1.06998 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 65.724, 65.724, 161.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.885 - 2.065 |
Rwork | 0.196 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eto |
RMSD bond length | 0.006 |
RMSD bond angle | 1.501 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.886 | 2.296 |
High resolution limit [Å] | 2.065 | 2.065 |
Rmerge | 0.105 | 1.431 |
Rmeas | 0.109 | 1.496 |
Rpim | 0.030 | 0.430 |
Number of reflections | 15144 | 757 |
<I/σ(I)> | 14.6 | |
Completeness [%] | 91.5 | 64.6 |
Redundancy | 12.9 | 11.8 |
CC(1/2) | 0.999 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2.0 M sodium chloride, 0.1 M sodium acetate pH 4.6 |