7AAM
Crystal structure of the F-BAR domain of PSTIPIP1 bound to the CTH domain of the phosphatase LYP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 105 |
Detector technology | PIXEL |
Collection date | 2015-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.996, 72.037, 205.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 102.520 - 2.150 |
R-factor | 0.2068 |
Rwork | 0.205 |
R-free | 0.24090 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7aan |
RMSD bond length | 0.001 |
RMSD bond angle | 0.324 |
Data reduction software | XDS (Jan 21, 2020) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.520 | 2.316 |
High resolution limit [Å] | 2.150 | 2.154 |
Rmerge | 0.397 | 3.036 |
Rmeas | 0.407 | |
Rpim | 0.722 | |
Number of reflections | 20328 | 1016 |
<I/σ(I)> | 8 | 1.4 |
Completeness [%] | 51.5 | 13.4 |
Redundancy | 19.1 | 17.8 |
CC(1/2) | 0.995 | 0.521 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM Bis-Tris-propane (pH=6.0), 20% (w/v) PEG 3350, 200 mM sodium citrate. LYP-CTH peptide was soaked into preformed crystals of the F-BAR domain of PSTPIP1. |