7AA4

Structure of ClpC1-NTD bound to a CymA analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-05-03
Detector	MARRESEARCH
Wavelength(s)	1.5406
Spacegroup name	P 1
Unit cell lengths	31.350, 33.680, 35.810
Unit cell angles	86.18, 94.22, 103.18

Refinement procedure

Resolution	24.770 - 1.680
R-factor	0.1714
Rwork	0.170
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdb
RMSD bond length	0.006
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.720
High resolution limit [Å]	1.680	1.680
Rmeas	0.062	0.112
Number of reflections	15176	1015
<I/σ(I)>	22.6	12.5
Completeness [%]	93.8	86.5
Redundancy	5.8	5.4
CC(1/2)	0.998	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES/imidazole pH 6.5, 6% (w/v) PEG 20K, 2% (v/v) PEG MME 550, 120 mM 1,6-hexanediol, 120 mM (RS)-1,2-propanediol, 120 mM 2-propanol, 120 mM 1,4-butanediol