7AA0
Structural comparison of cellular retinoic acid binding protein I and II in the presence and absence of natural and synthetic ligands
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2018-03-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.550, 49.920, 67.380 |
Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
Resolution | 40.064 - 1.820 |
Rwork | 0.197 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ogb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.550 |
Data reduction software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.920 | 49.920 | 1.860 |
High resolution limit [Å] | 1.820 | 9.100 | 1.820 |
Rmerge | 0.065 | 0.026 | 0.735 |
Rmeas | 0.089 | 0.037 | 1.003 |
Rpim | 0.061 | 0.026 | 0.678 |
Number of reflections | 23023 | 604 | 4918 |
<I/σ(I)> | 10.4 | ||
Completeness [%] | 97.6 | ||
Redundancy | 3.5 | 2.9 | 3.7 |
CC(1/2) | 0.996 | 0.998 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG based |