7A9X
Structure of yeast Rmd9p in complex with 16nt target RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 106.250, 106.250, 128.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.010 - 2.450 |
R-factor | 0.2004 |
Rwork | 0.199 |
R-free | 0.23270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7a9w |
RMSD bond length | 0.002 |
RMSD bond angle | 0.429 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.020 | 2.500 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.077 | 2.809 |
Number of reflections | 59634 | 4420 |
<I/σ(I)> | 18.11 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.7 | 10.25 |
CC(1/2) | 1.000 | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293.15 | Na-Cacodylate, NaCl, LiSO4, CaAcetate, MgCl2, PEG8000 |