7A8U

Crystal structure of sarcomeric protein FATZ-1 (d91-FATZ-1 construct) in complex with rod domain of alpha-actinin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979136
Spacegroup name	P 31 2 1
Unit cell lengths	101.650, 101.650, 218.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.440 - 3.802
R-factor	0.2175
Rwork	0.216
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a8t
RMSD bond length	0.007
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.400	3.900
High resolution limit [Å]	3.800	3.800
Rmerge	0.101	1.253
Number of reflections	13246	968
<I/σ(I)>	8.1	0.9
Completeness [%]	98.4	97
Redundancy	4.1	4.2
CC(1/2)	0.999	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	100 mM Tris-HCl (pH 8.5), 200 mM trimethylamine N-oxide, 20% w/v polyethylene glycol 2,000 methyl ether