7A6S
Crystal Structure of Asn173Ser variant of Human Deoxyhypusine Synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.382, 105.382, 159.902 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.030 - 1.750 |
R-factor | 0.1624 |
Rwork | 0.162 |
R-free | 0.19890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xxj |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.030 | 46.030 | 1.860 |
High resolution limit [Å] | 1.750 | 5.220 | 1.750 |
Rmerge | 0.120 | 0.067 | 2.359 |
Rmeas | 0.133 | 0.075 | 2.618 |
Total number of observations | 1050137 | ||
Number of reflections | 200356 | 7557 | 32299 |
<I/σ(I)> | 7.2 | 24.55 | 0.63 |
Completeness [%] | 99.9 | 99.8 | 99.5 |
Redundancy | 5.241 | 5.159 | 5.285 |
CC(1/2) | 0.996 | 0.994 | 0.385 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.025-0.125 mM carboxylic acid mix, 30-60% precipitant mix (MPD, PEG 3350, PEG 1000), 100 mM Tris-Bicine pH = 8.5 |