7A6O
Crystal Structure of the Complex of the Recombinant Von Willebrand Factor AIM-A1 domain and VHH81 at 2.1 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 283 |
Detector technology | PIXEL |
Collection date | 2020-02-15 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 65.234, 65.234, 233.231 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.800 - 2.117 |
Rwork | 0.195 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1auq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.727 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.900 | 2.300 |
High resolution limit [Å] | 2.117 | 2.117 |
Rmerge | 0.048 | 0.645 |
Number of reflections | 19344 | 2527 |
<I/σ(I)> | 15.1 | 1.7 |
Completeness [%] | 92.4 | 69.2 |
Redundancy | 5.2 | |
CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 283 | 3.2 M ammonium sulphate, 0.08 M sodium citrate |