7A68
proteinase K crystallized from 0.5 M NaNO3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER X8 PROTEUM |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.320, 68.320, 108.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.900 - 2.550 |
R-factor | 0.1496 |
Rwork | 0.147 |
R-free | 0.19980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2id8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.827 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.117 | 0.350 |
Rmeas | 0.130 | 0.387 |
Number of reflections | 8092 | 1199 |
<I/σ(I)> | 5.8749 | 2 |
Completeness [%] | 92.0 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Protein concentration: 10mg/ml. 0,2 M Tris pH 8, 0.5 M NaNO3 |