2ID8

Crystal structure of Proteinase K

Summary for 2ID8

• Entry DOI: 10.2210/pdb2id8/pdb
• Descriptor: Proteinase K, CALCIUM ION, NITRATE ION, ... (6 entities in total)
• Functional Keywords: proteinase, hydrolase
• Biological source: Engyodontium album
• Total number of polymer chains: 1
• Total formula weight: 29373.26

Authors

Wang, J.,Dauter, M.,Dauter, Z. (deposition date: 2006-09-14, release date: 2006-10-10, Last modification date: 2024-10-30)

Primary citation

Wang, J.,Dauter, M.,Dauter, Z.
What can be done with a good crystal and an accurate beamline?
Acta Crystallogr.,Sect.D, 62:1475-1483, 2006

PubMed Abstract: X-ray single-wavelength anomalous diffraction (SAD) data from a crystal of proteinase K were collected using synchrotron radiation of 0.98 A wavelength at SER-CAT 22-ID beamline, Advanced Photon Source, Argonne National Laboratory. At this wavelength, the expected Bijvoet ratio resulting from the presence of one calcium, one chloride and ten S atoms in the 279-residue protein is extremely small at approximately 0.46%. The direct-methods program SHELXD located 11 anomalous sites using data truncated to 2 A resolution. SHELXE was used to produce an easily interpretable electron-density map. This study shows that an accurate beamline and a good-quality crystal provide the possibility of successfully using a very weak anomalous signal of sulfur measured at a short wavelength for phasing a protein structure, even if a small degree of radiation damage is present.

PubMed: 17139083
DOI: 10.1107/S0907444906038534

Experimental method

X-RAY DIFFRACTION (1.27 Å)