Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.960, 117.950, 216.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.176 - 2.438 |
| R-factor | 0.214105983061 |
| Rwork | 0.212 |
| R-free | 0.25729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e43 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.180 | 2.525 |
| High resolution limit [Å] | 2.438 | 2.438 |
| Rmerge | 0.168 | 1.828 |
| Rmeas | 0.174 | 1.907 |
| Number of reflections | 77982 | 4879 |
| <I/σ(I)> | 8.81 | 0.84 |
| Completeness [%] | 84.0 | 98 |
| Redundancy | 13 | 12.4 |
| CC(1/2) | 0.998 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 14% IN PEG 3350, 0.1 M IN PHOSPHATE BUFFER AT A PH OF 6.25 PROTEIN : IN HEPES 5 MM AND NACL 200MM CRYOPROTECTION IN 20% OF GLYCEROL AND 20 MM OF SODIUM DITHIONITE, PH 7.6 |
