7A5T
Crystal structure of A55E mutant of BlaC from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.990, 54.356, 53.687 |
| Unit cell angles | 90.00, 91.94, 90.00 |
Refinement procedure
| Resolution | 38.210 - 1.400 |
| R-factor | 0.14896 |
| Rwork | 0.148 |
| R-free | 0.17725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gdn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.781 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.210 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rpim | 0.078 | 0.344 |
| Number of reflections | 42718 | 2050 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 96.9 | 94.2 |
| Redundancy | 2.5 | 2.3 |
| CC(1/2) | 0.985 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8 M Disodium Succinate |
