7A3P
Crystal structure of dengue 3 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.744, 101.412, 249.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 3.190 |
| R-factor | 0.243 |
| Rwork | 0.240 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DENV1 sE from related PDB |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.590 | 3.370 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.171 | 0.476 |
| Number of reflections | 23920 | 2139 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 87.3 | 56.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 100 mM HEPES pH 7.5, 15% PEG 8,000 |
