7A0W
Structure of dimeric sodium proton antiporter NhaA, at pH 8.5, crystallized with chimeric Fab antibodies
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 112.788, 92.163, 139.105 |
Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.860 | 2.340 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.087 | 1.680 |
Number of reflections | 94893 | 4739 |
<I/σ(I)> | 16 | 1.8 |
Completeness [%] | 94.1 | 67.6 |
Redundancy | 13.1 | 11.1 |
CC(1/2) | 0.999 | 0.428 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 100 mM Tris pH 8.5, 100 mM MgCl2, 100 mM NaCl, 32 % PEG400 |