7A0S
50S Deinococcus radiodurans ribosome bounded with mycinamicin I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-02-19 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.995, 410.736, 697.778 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.670 - 3.220 |
R-factor | 0.2252 |
Rwork | 0.224 |
R-free | 0.25340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pio |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.12_2829) |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.270 |
High resolution limit [Å] | 3.210 | 8.700 | 3.210 |
Rmerge | 0.182 | 0.064 | 1.574 |
Rmeas | 0.207 | 0.075 | 1.784 |
Rpim | 0.096 | 0.038 | 0.823 |
Total number of observations | 1739141 | ||
Number of reflections | 389019 | 19899 | 19343 |
<I/σ(I)> | 4.7 | ||
Completeness [%] | 99.6 | 98.1 | 99.6 |
Redundancy | 4.5 | 3.8 | 4.5 |
CC(1/2) | 0.995 | 0.448 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Ribosome solution containing 0.0065 mM (180 A/ml) of D50S in 10 mM Hepes pH=7.8, 15 mM MgCl2 and 75 mM NH4Cl crystallization buffer was mixed with 10 mM spermidine, 1 % ethanol and 0.5 % 2-ethyl-1,3-hexanediol precipitants. A 0.005 ml crystallization drop was hanged over 10 % ethanol and 5% 2-ethyl-1,3-hexanediol in ddw reservoir. |