7ZVX
Crystal structure of human Annexin A2 in complex with full phosphorothioate 5-10 2'-methoxyethyl DNA gapmer antisense oligonucleotide solved at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.230, 60.360, 128.430 |
| Unit cell angles | 90.00, 97.17, 90.00 |
Refinement procedure
| Resolution | 35.850 - 2.400 |
| R-factor | 0.1881 |
| Rwork | 0.185 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lpu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS (v3) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.470 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 29215 | 4021 |
| <I/σ(I)> | 8.96 | |
| Completeness [%] | 96.5 | |
| Redundancy | 6.9 | |
| CC(1/2) | 1.000 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 290 | 12% w/v PEG 8000, 24% v/v ethylene glycol, 0.02 M sodium formate/ammonium acetate/trisodium citrate/sodium potassium L-tartrate/sodium oxamate, 0.1 M MES/imidazole pH 6.1 |
