7ZVB
Crystal Structure of the mature form of the glutamic-class prolyl-endopeptidase neprosin at 2.35 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0050 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.760, 49.950, 59.860 |
| Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
| Resolution | 52.490 - 2.350 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zu8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 2.434 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 13077 | 1292 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.6 | 98.85 |
| Redundancy | 6.46 | |
| CC(1/2) | 0.990 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 1000, 10% PEG 8000 |
