7ZRY

Structure of the 2a splicing variant of the full-length human LSD1 bound to CoREST (delta305)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999998
Spacegroup name	I 2 2 2
Unit cell lengths	119.755, 179.747, 234.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.120 - 2.700
R-factor	0.221
Rwork	0.220
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	2.760
High resolution limit [Å]	2.700	2.700
Rmerge	0.102	3.105
Number of reflections	69570	701
<I/σ(I)>	12.8	0.68
Completeness [%]	100.0	98.9
Redundancy	6.9	7.2
CC(1/2)	0.999	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.0-1.3 M Na/K Tartrate, 0.1 M ADA pH 6.5