7ZOR

Crystal structure of anti-Siglec-15 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	127.020, 60.681, 131.258
Unit cell angles	90.00, 107.86, 90.00

Refinement procedure

Resolution	62.467 - 3.933
R-factor	0.2319
Rwork	0.229
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vkk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.467	4.001
High resolution limit [Å]	3.933	3.933
Rmerge	0.465	0.931
Rmeas	0.511	1.090
Rpim	0.208	0.552
Number of reflections	8960	429
<I/σ(I)>	6.4	3.2
Completeness [%]	99.9	99.9
Redundancy	6.1	4.4
CC(1/2)	0.865	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% (w/v) PEG3350 0.1M HEPES pH7.5 0.2M Magnesium chloride