7ZMQ
Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G2*-006
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9119 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 115.130, 199.086, 174.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.665 - 2.700 |
Rwork | 0.225 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lb5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.534 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.680 | 2.450 |
High resolution limit [Å] | 2.410 | 2.410 |
Number of reflections | 77373 | 3878 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 | |
CC(1/2) | 0.900 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M lithium sulfate -- 25% PEG3350 -- 0.1M tris pH 8.5 |