7ZML
Crystal structure of human RECQL5 helicase APO form in complex with engineered nanobody (Gluebody) G1-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.740, 185.530, 101.010 |
| Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
| Resolution | 96.351 - 2.790 |
| Rwork | 0.224 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lb5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.432 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.351 | 2.840 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmeas | 0.083 | |
| Number of reflections | 65889 | 3266 |
| <I/σ(I)> | 12.9 | 1.1 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 293 | 0.1M bis-tris pH 5.9 -- 0.1M lithium sulfate -- 30% PEG3350 |
