7ZJU

Structure of human USPL1 in covalent complex with SUMO3-2Br probe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	66.060, 84.833, 71.106
Unit cell angles	90.00, 92.26, 90.00

Refinement procedure

Resolution	54.470 - 2.170
R-factor	0.1942
Rwork	0.192
R-free	0.23780
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.640
Data reduction software	xia2 (0.5.902)
Data scaling software	Aimless (1.12.2)
Phasing software	CRANK2 (2.0.251)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.470	2.250
High resolution limit [Å]	2.170	2.170
Rmerge	0.112	0.640
Rmeas	0.123	0.711
Number of reflections	80896	3592
<I/σ(I)>	8.5	2.4
Completeness [%]	99.9	99.6
Redundancy	5.7	5
CC(1/2)	0.996	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	100 mM Tris pH 8.8, 19% PEG6000, 200 mM CaCl2