7ZJS

Structural basis of centromeric cohesion protection by SGO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-25
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	78.808, 181.098, 111.374
Unit cell angles	90.00, 94.25, 90.00

Refinement procedure

Resolution	47.870 - 3.240
R-factor	0.2699
Rwork	0.269
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qnx
RMSD bond length	0.006
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.870	3.350
High resolution limit [Å]	3.240	3.240
Number of reflections	48451	4517
<I/σ(I)>	8.1
Completeness [%]	99.8
Redundancy	2.7
CC(1/2)	0.990	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.06 M Morpheus Divalents mix, 0.1 M Morpheus buffer system 2, 48% (v/v) Morpheus EOD_P8K