7ZJF

R399E, a mutated form of GDF5, for disease modification of osteoarthritis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	36.426, 84.732, 98.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.220 - 1.300
R-factor	0.2077
Rwork	0.207
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bhk
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	1.380
High resolution limit [Å]	1.300	1.300
Rmeas	0.069
Rpim	0.023	0.436
Number of reflections	75754	11997
<I/σ(I)>	11.6
Completeness [%]	99.9	99.2
Redundancy	6.5	6.1
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.15	293	01 M Hepes, 32% PEG 400, 230 mM MgCl2, pH 8.15