7ZF4

SARS-CoV-2 Omicron RBD in complex with Omi-9 Fab and nanobody F2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-31
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	86.574, 205.147, 123.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.530 - 4.180
R-factor	0.3702
Rwork	0.369
R-free	0.38530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qnw
RMSD bond length	0.008
RMSD bond angle	1.130
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.000	4.250
High resolution limit [Å]	4.180	4.180
Rmerge	0.728
Rpim	0.207
Number of reflections	8472	410
<I/σ(I)>	2.5	0.4
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.877	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium acetate trihydrate pH 4.5 and 30% (w/v) PEG 1500