7ZEE

Human cytosolic 5' nucleotidase IIIB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	41.715, 79.288, 87.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.240 - 1.360
R-factor	0.1605
Rwork	0.159
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bdu
RMSD bond length	0.012
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.240	1.405
High resolution limit [Å]	1.357	1.357
Rmerge	0.052
Rmeas	0.055
Rpim	0.016
Number of reflections	60466	4228
<I/σ(I)>	25.78	1.68
Completeness [%]	95.2
Redundancy	11.9
CC(1/2)	0.999	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	277.15	0.1 M MES pH 6.2, 27% PEG 3350