7ZA3
GPC3-Unc5D octamer structure and role in cell migration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-09 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.80000 |
Spacegroup name | P 31 |
Unit cell lengths | 119.577, 119.577, 257.936 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 80.878 - 4.000 |
Rwork | 0.355 |
R-free | 0.38460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ftt GPC3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.375 |
Data reduction software | autoPROC |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.878 | 4.227 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.053 | |
Rpim | 0.020 | |
Number of reflections | 26038 | 1287 |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 73.8 | |
Redundancy | 8.2 | |
CC(1/2) | 1.000 | 0.761 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.8 | 291.15 | 0.1M Tris (pH 7.8) 5% w/v gamma-PGA, 20% w/vPEG 3350 |