7Z7D

Tubulin-Todalam-Vinblastine-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	105.286, 157.296, 182.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.600 - 2.000
R-factor	0.2054
Rwork	0.204
R-free	0.23570
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.080
High resolution limit [Å]	2.000	2.000
Rmerge	0.072	1.274
Rmeas	0.081	1.437
Rpim	0.036	0.647
Number of reflections	405947	64331
<I/σ(I)>	11.6	1.02
Completeness [%]	96.2
Redundancy	4.6
CC(1/2)	0.998	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine