7Z75

PI3KC2a core in complex with PITCOIN3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	56.064, 134.412, 152.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.010 - 2.590
R-factor	0.2254
Rwork	0.223
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bi4
RMSD bond length	0.013
RMSD bond angle	1.878
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	2.688
High resolution limit [Å]	2.590	2.595
Number of reflections	36105	3460
<I/σ(I)>	9.98
Completeness [%]	98.9
Redundancy	5.4
CC(1/2)	0.998	0.251

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	0.1MTris, 8-6% PEG3350, 0.2-0.1M MgSO4