7Z73
Crystal structure of p63 tetramerization domain in complex with darpin 8F1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-22 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.563, 50.761, 65.858 |
| Unit cell angles | 80.93, 79.92, 68.75 |
Refinement procedure
| Resolution | 47.060 - 2.270 |
| R-factor | 0.1896 |
| Rwork | 0.187 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zuv 4a9z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.060 | 47.060 | 2.350 |
| High resolution limit [Å] | 2.270 | 8.790 | 2.270 |
| Rmerge | 0.065 | 0.032 | 0.542 |
| Rmeas | 0.076 | 0.038 | 0.628 |
| Rpim | 0.038 | 0.019 | 0.318 |
| Total number of observations | 82692 | 1511 | 8112 |
| Number of reflections | 21523 | 397 | 2091 |
| <I/σ(I)> | 11.3 | 30 | 2.2 |
| Completeness [%] | 92.6 | 98.8 | 91.5 |
| Redundancy | 3.8 | 3.8 | 3.9 |
| CC(1/2) | 0.997 | 0.995 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M bis-tris pH 5.5 |
