7Z5A

Crystal structure of the trapped complex of mouse Endonuclease VIII-LIKE 3 (mNEIL3) and hairpin DNA with 5'overhang

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	56.117, 71.528, 94.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.130 - 2.280
R-factor	0.238
Rwork	0.237
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w0f
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.130	2.341
High resolution limit [Å]	2.260	2.260
Rmerge	0.190	0.320
Rmeas	0.201
Number of reflections	179306	1800
<I/σ(I)>	9.11
Completeness [%]	100.0
Redundancy	5.89
CC(1/2)	0.992	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	50 mM PIPES pH = 7.5 4% (w/v) PEG 8000 20 mM MgCl2 1 mM Spermine