7Z3W
Crystal structure of the AAL160 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-18 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.7998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.173, 89.832, 123.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.380 - 2.000 |
| R-factor | 0.1979 |
| Rwork | 0.196 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MIM.PDB |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 (1.96.1) |
| Data scaling software | SCALEPACK (1.96.1) |
| Phasing software | AMoRE (3.4) |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.000 | 28.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.050 | 0.033 | 0.381 |
| Number of reflections | 47440 | 5003 | 4670 |
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.9 | 99.7 | 100 |
| Redundancy | 8.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1M CHES pH 9.5, 50% (w/v) PEG 200 |
