7Z36

Crystal structure of the KAP1 tripartite motif in complex with the ZNF93 KRAB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	190.283, 68.583, 149.111
Unit cell angles	90.00, 114.05, 90.00

Refinement procedure

Resolution	63.790 - 2.800
R-factor	0.2291
Rwork	0.227
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qaj
RMSD bond length	0.013
RMSD bond angle	1.551
Data reduction software	xia2 (3.4.dev)
Data scaling software	DIALS (3.dev.431-gf865f6176)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.790	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.075	0.503
Rpim	0.052	0.343
Number of reflections	42537	4206
<I/σ(I)>	20.87	2.86
Completeness [%]	97.3	97.29
Redundancy	2.8	2.9
CC(1/2)	0.985	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	11% (w/v) PEG 5000 MME 5% Tacsimate 0.1 M HEPES pH 7