7Z27

Crystal structure of the SPOC domain of human RBM15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	45.031, 65.980, 116.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.450 - 1.450
R-factor	0.1983
Rwork	0.197
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7z1k
RMSD bond length	0.006
RMSD bond angle	0.881
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.450	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.112	2.080
Rmeas	0.116	2.120
Number of reflections	62616	4578
<I/σ(I)>	12.9
Completeness [%]	100.0
Redundancy	3.5
CC(1/2)	0.999	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2M Sodium malonate dibasic monohydrate, 0.1M Bis-Tris propane 6.5, 20% w/v PEG 3350