7YZW

Llama nanobody VHH15 raised against the glycoprotein of Ebola virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97857
Spacegroup name	P 64 2 2
Unit cell lengths	142.671, 142.671, 160.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.240 - 1.600
R-factor	0.1911
Rwork	0.190
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bnw
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (16-JUL-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	126134	18171
<I/σ(I)>	14.6
Completeness [%]	100.0
Redundancy	20.1
CC(1/2)	1.000	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	0,1 M CAPS pH10.5 0,2 M Lithium sulfate 1,2 M sodium phosphate monobasic 0,8 M potassium phosphate dibasic