7YZ5
Molecular snapshots of drug release from tubulin: 100 milliseconds (steady state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-10-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.280, 91.870, 83.690 |
Unit cell angles | 90.00, 96.82, 90.00 |
Refinement procedure
Resolution | 73.750 - 2.110 |
R-factor | 0.1757 |
Rwork | 0.173 |
R-free | 0.23080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nqt |
Data reduction software | CrystFEL (0.9.1) |
Data scaling software | CrystFEL (0.9.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.110 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 38945 | 117 |
<I/σ(I)> | 7.87 | 1.25 |
Completeness [%] | 60.0 | |
Redundancy | 274.2 | |
CC(1/2) | 0.992 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Ammonium sulfate, 0.1M Bis/Tris-methane pH=5.5, 21% PEG 3000 (m/v) |