7YXW
Structure of the p22phox A200G mutant in complex with p47phox peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.444, 102.444, 56.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ng2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.811 |
| Data reduction software | XDS (VERSION January 10, 2014) |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.810 | 45.810 | 2.600 |
| High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
| Rmerge | 0.098 | 0.048 | 1.141 |
| Rmeas | 0.104 | 0.051 | 1.192 |
| Rpim | 0.031 | 0.015 | 0.338 |
| Total number of observations | 2844 | 15254 | |
| Number of reflections | 10811 | 283 | 1181 |
| <I/σ(I)> | 11.8 | 26.8 | 1.7 |
| Completeness [%] | 99.9 | 98.7 | 99.9 |
| Redundancy | 12 | 10 | 12.9 |
| CC(1/2) | 0.997 | 0.999 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M HEPES pH 7.5, 1.2 M sodium citrate tribasic dihydrate, 1:1 protein:precipitant ratio |
