7YXO
Crystal structure of WT AncGR2-LBD bound to dexamethasone and SHP coregulator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 180.200, 180.200, 169.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.100 - 2.990 |
| R-factor | 0.2437 |
| Rwork | 0.242 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ufs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.555 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.100 | 3.180 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmeas | 0.307 | |
| Rpim | 0.149 | 0.815 |
| Number of reflections | 23704 | 4229 |
| <I/σ(I)> | 6.6 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.4 | 9.5 |
| CC(1/2) | 0.982 | 0.386 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M Tris-HCl, pH 8.0, 0.2 M sodium chloride, 2.0 M ammonium sulfate |
