7YXN
Crystal structure of WT AncGR2-LBD bound to dexamethasone and SHP coregulator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 109.479, 109.479, 137.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.810 - 2.460 |
| R-factor | 0.2177 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ufs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.810 | 2.560 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmeas | 0.074 | |
| Rpim | 0.051 | 0.725 |
| Number of reflections | 33009 | 3799 |
| <I/σ(I)> | 7.4 | 0.7 |
| Completeness [%] | 96.8 | 98.5 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.994 | 0.317 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.1 M PIPES, pH 7.0, 0.1 M ammonium acetate, 2.5 M sodium formate |
