7YXD
Crystal structure of WT AncGR2-LBD bound to dexamethasone and SHP coregulator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 |
| Unit cell lengths | 107.599, 107.599, 135.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.810 - 2.300 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ufs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.810 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.125 | 0.987 |
| Rpim | 0.079 | 0.641 |
| Number of reflections | 73686 | 10882 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 94.4 | 95.6 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.986 | 0.314 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M Tris-HCl, pH 8.0, 0.2 M sodium chloride, 2.0 M ammonium sulfate |
