7YVZ

Structure of Caenorhabditis elegans CISD-1/mitoNEET

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-08
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	37.798, 37.798, 82.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.350 - 1.700
R-factor	0.1699
Rwork	0.168
R-free	0.18910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	rat mitoNEET
RMSD bond length	0.015
RMSD bond angle	1.461
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.1192.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.350	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.049	0.472
Rmeas	0.053	0.508
Rpim	0.020	0.185
Number of reflections	7076	686
<I/σ(I)>	19.83	3.5
Completeness [%]	99.8
Redundancy	7.1	7.3
CC(1/2)	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM CHES-NaOH, pH 9.5, and 30%(w/v) PEG 3000 to which 5%(v/v) of 1 M Bicine, pH8.5, was added