7YO8
Crystal structure of fission yeast Hfl1 LIR fused to human GABARAPL2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 45.850, 45.850, 152.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.910 - 1.805 |
R-factor | 0.1785 |
Rwork | 0.177 |
R-free | 0.20540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4co7 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.774 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.19.2) |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.910 | 43.910 | 1.910 |
High resolution limit [Å] | 1.805 | 5.380 | 1.805 |
Rmerge | 0.154 | 0.087 | 1.287 |
Rmeas | 0.161 | 0.093 | 1.389 |
Rpim | 0.045 | ||
Number of reflections | 15809 | 1077 | 4612 |
<I/σ(I)> | 11 | 19.02 | 1.52 |
Completeness [%] | 99.9 | 99.9 | 99 |
Redundancy | 12.7 | 7.277 | 6.972 |
CC(1/2) | 0.997 | 0.995 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 8% PEG 3000, 0.1 M sodium citrate |