7YMR

Complex structure of lysoplasmalogen specific phopholipase D, F211L mutant with LPC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-21
Detector	RAYONIX MX225-HS
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	260.532, 260.532, 76.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.380 - 2.690
R-factor	0.2172
Rwork	0.216
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pz0
RMSD bond length	0.010
RMSD bond angle	1.165
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	2.750
High resolution limit [Å]	2.690	2.690
Number of reflections	73238	4425
<I/σ(I)>	25.7
Completeness [%]	99.9
Redundancy	29.6
CC(1/2)	1.000	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Sodium Chloride, HEPES, Ammonium Sulfate, LPC