7YIV

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (ALPL) at Basic pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	158.622, 167.348, 188.783
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.050 - 3.180
R-factor	0.1737
Rwork	0.172
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ew2
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.490	3.260
High resolution limit [Å]	3.170	3.200
Number of reflections	83898	11103
<I/σ(I)>	2.28
Completeness [%]	98.6	98.5
Redundancy	6.7	6.5
CC(1/2)	0.980	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	17% (w/v) PEG4000, 30 mM Tris, pH 8.0, 100 mM MgCl2