7YIT
Molecular mechanism of biased signaling at the kappa opioid receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 83 |
| Detector technology | PIXEL |
| Collection date | 2018-08-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0330 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 70.750, 76.530, 154.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.140 - 3.300 |
| R-factor | 0.2899 |
| Rwork | 0.285 |
| R-free | 0.33520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b73 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.380 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 40881 | 1594 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 94.5 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.990 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.7 | 293 | 40-200 mM magnesium sulfate hydrate, 100 mM sodium citrate tribasic dehydrate, 10 mM Manganese (II) chloride tetrahydrate 28-30% PEG400 |
