7YCK
Crystal structure of SARS-CoV-2 Spike RBD in complex with FP-12A Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-03-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.830, 79.124, 92.539 |
| Unit cell angles | 76.26, 81.16, 71.13 |
Refinement procedure
| Resolution | 32.250 - 2.600 |
| R-factor | 0.2302 |
| Rwork | 0.229 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zer |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.510 | 45.510 | 2.690 |
| High resolution limit [Å] | 2.600 | 10.070 | 2.600 |
| Rmerge | 0.093 | 0.051 | 0.917 |
| Rmeas | 0.107 | 0.059 | 1.059 |
| Rpim | 0.054 | 0.030 | 0.530 |
| Total number of observations | 183621 | 3028 | 18003 |
| Number of reflections | 46056 | 782 | 4515 |
| <I/σ(I)> | 5.6 | 9.8 | 1.4 |
| Completeness [%] | 97.8 | 96.5 | 97.1 |
| Redundancy | 4 | 3.9 | 4 |
| CC(1/2) | 0.995 | 0.987 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 0.2M Ammonium sulfate; 0.05 M Magnesium sulfate heptahydrate, 0.1M BICINE pH 9.0, 2.5% w/v PEG 3350, 2.5%w/v PEG 4000, 2.5% w/v PEG 2000, 2.5% w/v PEG 5000 MME |
