7YBX
Crystal structure of FGFR4(V550M) kinase domain with 10z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-05 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.139, 61.084, 60.619 |
| Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
| Resolution | 42.823 - 2.233 |
| R-factor | 0.1816 |
| Rwork | 0.180 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wct |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.680 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.823 | 2.313 |
| High resolution limit [Å] | 2.233 | 2.233 |
| Rmeas | 0.222 | |
| Number of reflections | 14877 | 1436 |
| <I/σ(I)> | 9.75 | 6.08 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 7.3 | 6.6 |
| CC(1/2) | 0.980 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350 |
