7YB7
anti-apoptotic protein BCL-2-M12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-01-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.761, 104.788, 111.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.520 - 2.200 |
R-factor | 0.17626 |
Rwork | 0.175 |
R-free | 0.19375 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.961 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
High resolution limit [Å] | 2.200 | 6.100 | 2.250 |
Rmerge | 0.104 | 0.062 | 0.432 |
Rmeas | 0.107 | 0.064 | 0.442 |
Rpim | 0.023 | 0.015 | 0.093 |
Number of reflections | 29689 | 886 | 742 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 20 | 20.1 | 22.3 |
CC(1/2) | 0.994 | 0.998 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.5 M Ammonium sulfate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Lithium sulfate monohydrate |